Semaglutide Reconstitution Calculator
Semaglutide is the most-studied GLP-1 receptor agonist. Research-grade Semaglutide ships lyophilized and is reconstituted with bacteriostatic water. Because the titration schedule starts at very small weekly doses (0.25 mg), precise concentration math is essential.
For laboratory research use only.
Peptide Reconstitution Calculator
Enter your vial size and bacteriostatic water volume. The calculator outputs the resulting concentration per mL and per insulin syringe unit. For procedural reference only.
Reading the result: If you have a 5mg vial reconstituted with 2mL of BAC water, your concentration is 2,500 mcg/mL. On a U-100 insulin syringe, that's 25 mcg per IU mark.
For laboratory research use only. This calculator is a procedural reference for concentration math. It does not constitute dosing advice. Researchers should consult published literature and qualified expertise for their research protocols.
Semaglutide concentration reference
Pre-computed concentrations for common Semaglutide vial sizes (3 mg, 5 mg, 10 mg) across 1–3 mL of bacteriostatic water. All values assume a U-100 insulin syringe.
| Vial | BAC water | Concentration | Per IU (U-100) |
|---|---|---|---|
| 3 mg | 1 mL | 3,000 mcg/mL | 30 mcg |
| 3 mg | 2 mL | 1,500 mcg/mL | 15 mcg |
| 3 mg | 3 mL | 1,000 mcg/mL | 10 mcg |
| 5 mg | 1 mL | 5,000 mcg/mL | 50 mcg |
| 5 mg | 2 mL | 2,500 mcg/mL | 25 mcg |
| 5 mg | 3 mL | 1,667 mcg/mL | 16.7 mcg |
| 10 mg | 1 mL | 10,000 mcg/mL | 100 mcg |
| 10 mg | 2 mL | 5,000 mcg/mL | 50 mcg |
| 10 mg | 3 mL | 3,333 mcg/mL | 33.3 mcg |
Semaglutide research reference
- Dosing range: Titration: 0.25 mg/wk weeks 1–4, escalating to 2.4 mg/wk maintenance (published manufacturer titration)
- Administration: Subcutaneous, once weekly
- Storage: Refrigerate after reconstitution; manufacturer guidance is 28 days post-reconstitution.
Ranges summarized from published research literature. Not medical or dosing advice.Semaglutide and all compounds referenced are for laboratory research use only — not for human consumption.
How the math works
Concentration (mcg/mL) = vial mass (mg) × 1000 ÷ bacteriostatic water volume (mL). For a 3 mg Semaglutide vial reconstituted with 1 mL, that is 3,000 mcg/mL. On a U-100 insulin syringe (100 IU marks per mL), each IU mark holds 30 mcg.
New to reconstitution? Read our complete reconstitution guide and what bacteriostatic water is. For Semaglutide specifically, see our Semaglutide research guide.
Before you reconstitute, verify your vial against its Semaglutide Certificate of Analysis. Sourcing a fresh vial? See where to buy Semaglutide — what to check before ordering and how the tested vendors compared.
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