NAD+ Reconstitution Calculator
NAD+ is studied in cellular-aging and longevity research. It ships lyophilized in large vials (100–500 mg) and is reconstituted with bacteriostatic water. NAD+ is hygroscopic and degrades faster than most peptides, so supplier cold-chain shipping and post-reconstitution storage matter more here.
For laboratory research use only.
Peptide Reconstitution Calculator
Enter your vial size and bacteriostatic water volume. The calculator outputs the resulting concentration per mL and per insulin syringe unit. For procedural reference only.
Reading the result: If you have a 5mg vial reconstituted with 2mL of BAC water, your concentration is 2,500 mcg/mL. On a U-100 insulin syringe, that's 25 mcg per IU mark.
For laboratory research use only. This calculator is a procedural reference for concentration math. It does not constitute dosing advice. Researchers should consult published literature and qualified expertise for their research protocols.
NAD+ concentration reference
Pre-computed concentrations for common NAD+ vial sizes (100 mg, 500 mg) across 1–3 mL of bacteriostatic water. All values assume a U-100 insulin syringe.
| Vial | BAC water | Concentration | Per IU (U-100) |
|---|---|---|---|
| 100 mg | 1 mL | 100,000 mcg/mL | 1,000 mcg |
| 100 mg | 2 mL | 50,000 mcg/mL | 500 mcg |
| 100 mg | 3 mL | 33,333 mcg/mL | 333.3 mcg |
| 500 mg | 1 mL | 500,000 mcg/mL | 5,000 mcg |
| 500 mg | 2 mL | 250,000 mcg/mL | 2,500 mcg |
| 500 mg | 3 mL | 166,667 mcg/mL | 1,666.7 mcg |
NAD+ research reference
- Dosing range: 100–500 mg per dose, daily or alternating days (published research range)
- Administration: Subcutaneous or intramuscular
- Storage: Most thermally fragile compound here. Refrigerate; typically used within 14 days reconstituted.
Ranges summarized from published research literature. Not medical or dosing advice.NAD+ and all compounds referenced are for laboratory research use only — not for human consumption.
How the math works
Concentration (mcg/mL) = vial mass (mg) × 1000 ÷ bacteriostatic water volume (mL). For a 500 mg NAD+ vial reconstituted with 5 mL, that is 100,000 mcg/mL. On a U-100 insulin syringe (100 IU marks per mL), each IU mark holds 1,000 mcg.
New to reconstitution? Read our complete reconstitution guide and what bacteriostatic water is. For NAD+ specifically, see our NAD+ research guide.
Before you reconstitute, verify your vial against its NAD+ Certificate of Analysis. Sourcing a fresh vial? See where to buy NAD+ — what to check before ordering and how the tested vendors compared.
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